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superscript iii reverse transcriptase kit  (Vazyme Biotech Co)


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    Vazyme Biotech Co superscript iii reverse transcriptase kit
    Superscript Iii Reverse Transcriptase Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 10390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+reverse+transcriptase+kit/HiScript+III+RT+SuperMix+for+qPCR+%2BgDNA+wiper/10__1016_slash_j__postharvbio__2025__113998-85-10-15
    Average 99 stars, based on 10390 article reviews
    superscript iii reverse transcriptase kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: VAPB-mediated ER-targeting stabilizes IRS-1 signalosomes to regulate insulin/IGF signaling.
    Article Snippet: .. As previously described86, total RNA was isolated using RNeasy Kit (Axygen, AP-MX-MS-RNA-250) and reverse transcription was performed using a SuperScript III reverse transcriptase kit (Vazyme, R223-01). qPCR reactions using SYBR qPCR Master Mix kit (Vazyme, R71102) were performed in triplicate for each gene with three independent samples prepared under the same conditions. ..

    Reverse Transcription:

    Article Title: VAPB-mediated ER-targeting stabilizes IRS-1 signalosomes to regulate insulin/IGF signaling.
    Article Snippet: .. As previously described86, total RNA was isolated using RNeasy Kit (Axygen, AP-MX-MS-RNA-250) and reverse transcription was performed using a SuperScript III reverse transcriptase kit (Vazyme, R223-01). qPCR reactions using SYBR qPCR Master Mix kit (Vazyme, R71102) were performed in triplicate for each gene with three independent samples prepared under the same conditions. ..

    Article Title: Suppression of ethylene metabolism serves as a key factor underlying high temperature-induced promotion of anthocyanin synthesis of peach skin
    Article Snippet: This study investigated the effects of high temperature (HT, 35C) on anthocyanin accumulation and ethylene metabolism in postharvest ‘Baifeng’ peaches, compared with control temperature (CT, 25C).. Results showed that HT significantly enhanced total anthocyanin content in peach skin by 1.55-fold at day 9, primarily attributed to upregulated expression of biosynthesis genes and transcription factors, as well as increased activities of key enzymes (PAL, CHS, UFGT).. Simultaneously, HT suppressed ethylene production to 12.18 % of CT levels by inhibiting the activities and gene expression of ethylene biosynthesis enzymes and downregulating ethylene signaling pathway.

    Article Title: Titanium Dioxide Nanoparticles Mitigated 2,4,6- Trinitrobenzenesulfonic (TNBS) Acid Solution Induced Colitis By Downregulating NF-κB Related Transcription
    Article Snippet: .. SuperScript III Reverse transcriptase kit (Vazyme Biotech Co.,Ltd, Nanjing, China) was further used for the complementary DNA (cDNA) synthesis, the collected cDNA was quanti ed by quantitative real time polymerase chain reaction (PCR, Bio-Rad iCycler iQ5, Bio-Rad Co.,Ltd, the United states). ..

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1, OsREM4.1, and Brd2 protein levels in brassinosteroid pathways in rice.
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401-01; Vazyme, Nanjing, China). .. The first-strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323-01; Vazyme). qRT-PCR analysis was performed on a CFX96 real-time PCR system (qTOWER3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μM of each primer and 1 × SYBR green qPCR master mix (Q711-03; Vazyme), and the Actin1 gene was used as the internal control.

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1 , OsREM4 .1, and Brd2 protein levels in brassinosteroid pathways in rice
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401‐01; Vazyme, Nanjing, China). .. The first‐strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323‐01; Vazyme). qRT‐PCR analysis was performed on a CFX96 real‐time PCR system (qTOWER 3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μ m of each primer and 1 × SYBR green qPCR master mix (Q711‐03; Vazyme), and the Actin1 gene was used as the internal control.

    Article Title: Development and Clinical Application of a Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for SARS-CoV-2 Infection
    Article Snippet: .. Briefly, total RNA was reverse transcribed to synthesize first-strand cDNA with random hexamers and a Superscript III reverse transcriptase kit (Vazyme, China). .. Two-step SARS-CoV-2 genome amplification was performed with two pooled mixtures of primer sets (designed by Genskey Medical Technology Co., Ltd.) designed to cover the entire SARS-CoV-2 genome. cDNA was mixed with the components of the first PCR according to the manufacturer’s instructions.

    Article Title: Development and Clinical Application of a Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for SARS-CoV-2 Infection
    Article Snippet: .. Briefly, total RNA was reverse transcribed to synthesize first-strand cDNA with random hexamers and a Superscript III reverse transcriptase kit (Vazyme, China). .. Two-step SARS-CoV-2 genome amplification was performed with two pooled mixtures of primer sets (designed by Genskey Medical Technology Co., Ltd.) designed to cover the entire SARS-CoV-2 genome. cDNA was mixed with the components of July/August 2020 Volume 5 Issue 4 e00808-20 msphere.asm.org 10 on A ugust 30, 2020 by guest http://m sphere.asm .org/ D ow nloaded from the first PCR according to the manufacturer’s instructions.

    Real-time Polymerase Chain Reaction:

    Article Title: VAPB-mediated ER-targeting stabilizes IRS-1 signalosomes to regulate insulin/IGF signaling.
    Article Snippet: .. As previously described86, total RNA was isolated using RNeasy Kit (Axygen, AP-MX-MS-RNA-250) and reverse transcription was performed using a SuperScript III reverse transcriptase kit (Vazyme, R223-01). qPCR reactions using SYBR qPCR Master Mix kit (Vazyme, R71102) were performed in triplicate for each gene with three independent samples prepared under the same conditions. ..

    Article Title: Titanium Dioxide Nanoparticles Mitigated 2,4,6- Trinitrobenzenesulfonic (TNBS) Acid Solution Induced Colitis By Downregulating NF-κB Related Transcription
    Article Snippet: .. SuperScript III Reverse transcriptase kit (Vazyme Biotech Co.,Ltd, Nanjing, China) was further used for the complementary DNA (cDNA) synthesis, the collected cDNA was quanti ed by quantitative real time polymerase chain reaction (PCR, Bio-Rad iCycler iQ5, Bio-Rad Co.,Ltd, the United states). ..

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1, OsREM4.1, and Brd2 protein levels in brassinosteroid pathways in rice.
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401-01; Vazyme, Nanjing, China). .. The first-strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323-01; Vazyme). qRT-PCR analysis was performed on a CFX96 real-time PCR system (qTOWER3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μM of each primer and 1 × SYBR green qPCR master mix (Q711-03; Vazyme), and the Actin1 gene was used as the internal control.

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1 , OsREM4 .1, and Brd2 protein levels in brassinosteroid pathways in rice
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401‐01; Vazyme, Nanjing, China). .. The first‐strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323‐01; Vazyme). qRT‐PCR analysis was performed on a CFX96 real‐time PCR system (qTOWER 3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μ m of each primer and 1 × SYBR green qPCR master mix (Q711‐03; Vazyme), and the Actin1 gene was used as the internal control.

    cDNA Synthesis:

    Article Title: Suppression of ethylene metabolism serves as a key factor underlying high temperature-induced promotion of anthocyanin synthesis of peach skin
    Article Snippet: This study investigated the effects of high temperature (HT, 35C) on anthocyanin accumulation and ethylene metabolism in postharvest ‘Baifeng’ peaches, compared with control temperature (CT, 25C).. Results showed that HT significantly enhanced total anthocyanin content in peach skin by 1.55-fold at day 9, primarily attributed to upregulated expression of biosynthesis genes and transcription factors, as well as increased activities of key enzymes (PAL, CHS, UFGT).. Simultaneously, HT suppressed ethylene production to 12.18 % of CT levels by inhibiting the activities and gene expression of ethylene biosynthesis enzymes and downregulating ethylene signaling pathway.

    Article Title: Titanium Dioxide Nanoparticles Mitigated 2,4,6- Trinitrobenzenesulfonic (TNBS) Acid Solution Induced Colitis By Downregulating NF-κB Related Transcription
    Article Snippet: .. SuperScript III Reverse transcriptase kit (Vazyme Biotech Co.,Ltd, Nanjing, China) was further used for the complementary DNA (cDNA) synthesis, the collected cDNA was quanti ed by quantitative real time polymerase chain reaction (PCR, Bio-Rad iCycler iQ5, Bio-Rad Co.,Ltd, the United states). ..

    Polymerase Chain Reaction:

    Article Title: Titanium Dioxide Nanoparticles Mitigated 2,4,6- Trinitrobenzenesulfonic (TNBS) Acid Solution Induced Colitis By Downregulating NF-κB Related Transcription
    Article Snippet: .. SuperScript III Reverse transcriptase kit (Vazyme Biotech Co.,Ltd, Nanjing, China) was further used for the complementary DNA (cDNA) synthesis, the collected cDNA was quanti ed by quantitative real time polymerase chain reaction (PCR, Bio-Rad iCycler iQ5, Bio-Rad Co.,Ltd, the United states). ..

    Synthesized:

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1, OsREM4.1, and Brd2 protein levels in brassinosteroid pathways in rice.
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401-01; Vazyme, Nanjing, China). .. The first-strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323-01; Vazyme). qRT-PCR analysis was performed on a CFX96 real-time PCR system (qTOWER3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μM of each primer and 1 × SYBR green qPCR master mix (Q711-03; Vazyme), and the Actin1 gene was used as the internal control.

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1 , OsREM4 .1, and Brd2 protein levels in brassinosteroid pathways in rice
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401‐01; Vazyme, Nanjing, China). .. The first‐strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323‐01; Vazyme). qRT‐PCR analysis was performed on a CFX96 real‐time PCR system (qTOWER 3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μ m of each primer and 1 × SYBR green qPCR master mix (Q711‐03; Vazyme), and the Actin1 gene was used as the internal control.

    Quantitative RT-PCR:

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1, OsREM4.1, and Brd2 protein levels in brassinosteroid pathways in rice.
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401-01; Vazyme, Nanjing, China). .. The first-strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323-01; Vazyme). qRT-PCR analysis was performed on a CFX96 real-time PCR system (qTOWER3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μM of each primer and 1 × SYBR green qPCR master mix (Q711-03; Vazyme), and the Actin1 gene was used as the internal control.



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